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Description:Under a moderately-high magnification of 8000X, this digitally-colorized scanning electron microscopic (SEM) image depicted a large grouping of Gram-negative, Legionella pneumophila bacteria. Please see PHIL 11092 through 11152 for additional SEMs of these organisms, specifically PHIL 11151 for a black and white version of this image. Of particular importance, is the presence of polar flagella, and pili, or long streamers, which due to their fragile nature, in some of these views seem to be dissociated from any of the bacteria.
You’ll note that a number of these bacteria seem to display an elongated-rod morphology. L. pneumophila are known to most frequently exhibit this configuration when grown in broth, however, they can also elongate when plate-grown cells age, as it was in this case, especially when they’ve been refrigerated. The usual L. pneumophila morphology consists of stout, “fat” bacilli, which is the case for the vast majority of the organisms depicted here. These bacteria originated on a 1 week-old culture plate (+/- 1 day), which had incubated a single colony, at 37oC upon a buffered charcoal yeast extract (BCYE) medium with no antibiotics.
High Resolution: Click here for hi-resolution image (16.43 MB)
Content Providers(s):CDC/ Margaret Williams, PhD; Claressa Lucas, PhD;Tatiana Travis, BS
Creation Date:2009
Photo Credit:Janice Haney Carr
Links:CDC – National Center for Immunization and Respiratory Diseases; Legionellosis Resource Site (Legionnaires' Disease and Pontiac Fever)
CDC Organization
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Copyright Restrictions:None - This image is in the public domain and thus free of any copyright restrictions. As a matter of courtesy we request that the content provider be credited and notified in any public or private usage of this image.