ID#: 3873
These Clostridium perfringens colonies were cultured on a half-antitoxin plate, in order to perform what is referred to as the Nagler reaction, or the lecithinase test. Antitoxin was added to the left side of this plate, thereby, inhibiting the lecithinase reaction, which occurred on the right, antitoxin-free region of the plate, where this reaction was allowed to occur, and where egg yolk lecithin was broken down to an opaque, insoluble diglyceride. Cl. perfringens produces lecithinase, and if present, as in this case, will produce the opacity in the egg yolk growth medium you see in this example.
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Content Provider(s): CDC/Dr. Stuart E. Starr
Creation Date: 1971
Photo Credit:
CDC Organization
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Copyright Restrictions: None - This image is in the public domain and thus free of any copyright restrictions.