ID#: 15509
Caption: Under a magnification of 500X, this photomicrograph of a biopsied lung tissue specimen, revealed small, blunt, pleomorphic intracellular, and extracellular bacilli, which stained brown to black against a yellow background. The specimen was processed using the Center for Disease Control (CDC) modified Dieterle silver impregnation procedure, which is used when Legionella pneumophila bacteria are suspected. Uranyl nitrate is applied in order to first sensitize the slide mount, which is subsequently treated with gum mastic, followed by an incubation period, after which the slide is soaked in silver nitrate. Lastly, the slide is “developed” in a hydroquinone, sodium sulfite, acetone, formaldehyde, pyridine, and gum mastic bath. The Legionella pneumophila bacteria, if present, will stain black.
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Content Providers(s): CDC
Creation Date: 1978
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Copyright Restrictions: None - This image is in the public domain and thus free of any copyright restrictions.