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ID#:15509
Description:Under a magnification of 500X, this micrograph of a biopsied lung tissue specimen was stained using the Center for Disease Control's modified Dieterle silver impregnation procedure, revealing small, blunt, pleomorphic intracellular, and extracellular bacilli, which stain brown to black against a yellow background; Mag. 500x.
Dieterle’s stain is a preparation used when Legionella pneumophila bacteria are suspected. Uranyl nitrate is applied in order to first sensitize the slide mount, which is subsequently treated with gum mastic, followed by an incubation period, after which the slide is soaked in silver nitrate. Lastly, the slide is “developed” in a hydroquinone, sodium sulfite, acetone, formaldehyde, pyridine, and gum mastic bath. The Legionella pneumophila bacteria, if present, will stain black.
High Resolution: Click here for hi-resolution image (18.34 MB)
Content Providers(s):CDC
Creation Date:1978
Photo Credit:
Links:CDC - National Center for Immunization and Respiratory Diseases; Legionellosis Resource Site (Legionnaires' Disease and Pontiac Fever); Patient Facts: Learn More about Legionnaires' disease
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Copyright Restrictions:None - This image is in the public domain and thus free of any copyright restrictions. As a matter of courtesy we request that the content provider be credited and notified in any public or private usage of this image.

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