This illustration outlines the contents of a genital mycoplasma transport tube, within which a transport medium is contained that would be inoculated with a specimen suspected of containing Mycoplasma spp. bacteria. Included in the culture broth are the following constituents:
- Pleuropneumonia-like Organism (PPLO) broth
- Yeast Extract
- Serum (horse)
- 0.4% Phenol Red
- Penicillin (100,000 U/ml)
- Polymyxin (5,000µg/ml)
- Amphotericin (5,000µg/ml). The red color of the broth mixture is imparted by the phenol which is a pH-sensitive color indicator.