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- Pleuropneumonia-like Organism (PPLO) broth
- Yeast Extract
- Serum (horse)
- 0.4% Phenol Red
- Penicillin (100,000 U/ml)
- Polymyxin (5,000µg/ml)
- Amphotericin (5,000µg/ml). The red color of the broth mixture is imparted by the phenol which is a pH-sensitive color indicator.
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