ID#: 14943
This image depicts a 96-well microtitration V-plate, which was being used to conduct a microtiter direct agglutination tests (DAT), upon immune serum involving organisms that included Leishmania donovani, L. tropica, and L. brasiliensis, with Evans blue dye having been added to each well. The test is conducted in the following manner. Into each well, a solution is dispensed, usually saline, along with a small amount of Evans blue dye, and serum suspected of containing antibodies to the Leishmania spp. organisms under consideration. Subsequently, to this mixture, which will vary quantitatively per each respective well, an antigen suspension, prepared from formalin-killed, promastigote stages of Leishmania spp. cultures is then added. If antibodies to the Leishmania spp. organisms are present, an agglutination reaction will occur.
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Content Provider(s): CDC/ Dorothy Allain
Creation Date: 1974
Photo Credit:
CDC Organization
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Copyright Restrictions: None - This image is in the public domain and thus free of any copyright restrictions.