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Description:This image depicts a 96-well microtitration ā€œVā€ plate, which was being used to conduct microtiter direct agglutination tests (DAT) upon immune serum involving organisms that included Leishmania donovani, L. tropica, and L. brasiliensis, with Evans blue dye having been added to each well.
Into each well, a solution is dispensed, usually saline, along with a small amount of Evans blue dye, and serum suspected of containing antibodies to the Leishmania spp. organisms under consideration. Subsequently, to this mixture, which will vary quantitatively per each respective well, an antigen suspension, prepared from formalin-killed promastigote stages of Leishmania spp. cultures is added. If antibodies to the Leishmania spp. organisms are present, an agglutination reaction will occur.
High Resolution: Click here for hi-resolution image (18.47 MB)
Content Providers(s):CDC/ Dorothy Allain
Creation Date:1974
Photo Credit:
Links:CDC - Global Health - Division of Parasitic Diseases and Malaria; Parasites: Leishmaniasis
CDC Organization
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Copyright Restrictions:None - This image is in the public domain and thus free of any copyright restrictions. As a matter of courtesy we request that the content provider be credited and notified in any public or private usage of this image.