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Centers for Disease Control and Prevention. CDC twenty four seven. Saving Lives, Protecting People
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At the component level, this diagram illustrates what takes place microbiologically during an indirect hemagglutination (IHA) inhibition test, which implements the herpes simplex virus (HSV) antigen in order to detect the presence of the HSV-specific antibody. Note that the upper portion of the diagram illustrates the negative interaction between the HSV-specific antibody, and a heterologous antigen, leaving the HSV antibody free to subsequently bind to sheep red blood cells (RBCs) that had been sensitized with HSV antigen. Had the HSV-specific antigen bound to the heterologous antigen, no hemagglutination of the sheep RBCs would have occurred. The results of this test indicate that there was no HSV antigen present in the initial sample, prior to its indirect addition by way of the sensitized sheep RBCs.
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CDC/ Dr. F.T. Forrester
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