|Description:||This photomicrograph reveals the presence of numerous Pneumocystis jirovecii, formerly Pneumocystis carinii, fungal organisms in a tissue sample, which had undergone the process of cellular sonication, also known as sonoporation. The technique of sonication is used to disrupt the cellular architecture in order to release, or separate out intracellular debris, or particles, which in this case, were these cystic-staged parasites.|
The specific diagnosis is based on identification of P. jirovecii in bronchopulmonary secretions obtained as induced sputum, or bronchoalveolar lavage (BAL) material. In situations where these two techniques cannot be used, transbronchial biopsy or open lung biopsy may prove necessary. Microscopic identification of P. jirovecii trophozoites and cysts is performed with stains that demonstrate either the nuclei of trophozoites and intracystic stages (such as Giemsa) or the cyst walls (such as the silver stains). In addition, immunofluorescence microscopy using monoclonal antibodies can identify the organisms with higher sensitivity than conventional microscopy.
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|Links:||CDC – Div. of Parasitic Diseases (DPDx); Pneumocystis infection|
CDC – Emerging Infectious Diseases, ”Pneumocystis jirovecii in General Population”, by Francisco J. Medrano, et. al., Vol. 11, No. 2 – February, 2005, Research
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