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ID#:11243
Description:This 1981 photograph depicted a number of Coplin jars ready for what would be a trichrome staining procedure.

Trichrome Staining Procedure

The trichrome stain is a simple and stable stain to use that renders organisms easier to detect because the nuclear chromatin and cytoplasm stain different colors, unlike iron hematoxylin which is a monochromatic stain. Staining containers such as Coplin jars arranged in a row make staining 1" × 3" slides systematic and simple when moving from one solution to another. Besides our example of trichrome staining of PVA-fixed specimens, one should also be aware that there are many other fixatives and staining procedures available.
Specimen:

The specimens usually consist of fresh stool or stool fixed in polyvinyl alcohol (PVA) smeared on microscope slides and allowed to air dry or dry on a slide warmer at 60°C. Stool preserved in sodium acetate-acetic acid-formalin (SAF) or some of the one-vial fixatives can also be used.

Reagents:

There are seven steps to this procedure, requiring the following solutions:

1.- 70% Ethanol plus iodine: prepare a stock solution by adding iodine crystals to 70% alcohol until you obtain a dark solution. To use, dilute the stock with 70% alcohol until a dark reddish brown color or strong tea color is obtained

2.- 70% Ethanol (twice)

3.- Trichrome Stain: may be purchased commercially

4.- 90% Acid Ethanol

    - 90% ethanol = 99.5 ml

    - Acetic acid (glacial) = 0.5 ml

5.- 95% ethanol

6.- 100% ethanol (twice)

7.- Xylene or xylene substitute (twice)

High Resolution: Click here for hi-resolution image (4.5 MB)
Content Providers(s):CDC/ Dr. Mae Melvin
Creation Date:1981
Photo Credit:
Links:CDC - Div. of Parasitic Diseases, DPDx; Selection of Standard Techniques for Examination of Fecal Specimens
CDC – Div. of Parasitic Diseases, DPDx; Stool Specimens: Staining Procedures
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Copyright Restrictions:None - This image is in the public domain and thus free of any copyright restrictions. As a matter of courtesy we request that the content provider be credited and notified in any public or private usage of this image.

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