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The S. pneumoniae bacterial inoculum were harvested from a primary nasopharyngeal, i.e., throat swab. The culture plate was stored under modified atmospheric conditions, by using a candle-extinction jar, thereby, bringing about an approximate 3% increase in carbon dioxide.
Note that the so called doughnut-shaped colonies are those of Streptococcus pneumoniae. Those that do not have depressed centers are not S. pneumoniae.
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