ID#: 3909
Caption:
This photograph depicts a close-up of what is referred to as a string test, which was being performed on a droplet of sample that was harvested from a Petri dish growing suspected colonies of Vibrio cholerae bacteria. Note the mucoid string still clinging to the wire inoculating loop, indicating a positive string test result. Mixing the bacterial sample with 3% potassium hydroxide (KOH), Gram-negative bacteria will lyse, and release their contents of sticky nuclei acids, which when touched by a wire loop, will cling together forming a stringy filament as the loop is lifted away from the slide. Gram-positive bacteria have a thicker cell wall, protected by a layer of peptidoglycan, thereby, able to resist the lytic action of the KOH reagent, and thus release no sticky nuclei acids, and hence, no string is produced.
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Content Provider(s): CDC
Creation Date: 1971
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Copyright Restrictions: None - This image is in the public domain and thus free of any copyright restrictions.