ID#: 22905
Caption:
This image depicted a Centers for Disease Control and Prevention (CDC) scientist interacting with her Caliper LifeSciences’ Zephyr Molecular Biology Workstation, working with samples to be tested using a real-time PCR machine, known as a themocycler (see PHIL 22904), in order to identify the various types of poliovirus contained therein. The data from this analysis is stored in a computer, while the software further analyzes the data before being reviewed by a scientist. The themocycler can vary the temperature, which is important, for PCR requires multiple test rounds at different temperatures. In the instrument, viral RNA is copied into DNA and then the DNA is amplified. Specific probes bind to the DNA, in order to determine what type of polio present. One hundred ten labs around the world can run this assay, and can tell if an isolate contains polio, or not, and if so, what kind.
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Content Provider(s): CDC/ Holly Patrick, MS, MPH
Creation Date: 2018
Photo Credit: James Gathany
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MeSH
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Analytical, Diagnostic and Therapeutic Techniques and Equipment
Diagnosis
Laboratory Techniques and Procedures
Investigative Techniques
Clinical Laboratory Techniques
Microbiological Techniques
Virus Cultivation
Molecular Diagnostic Techniques
Genetic Techniques
Nucleic Acid Amplification Techniques
Polymerase Chain Reaction
Chemicals and Drugs
Chemical Actions and Uses
Specialty Uses of Chemicals
Laboratory Chemicals
Culture Media
Diseases
Nervous System Diseases
Central Nervous System Diseases
Central Nervous System Infections
Central Nervous System Viral Diseases
Myelitis
Poliomyelitis
Poliomyelitis
Myelitis
Poliomyelitis
Spinal Cord Diseases
Myelitis
Poliomyelitis
Poliomyelitis
Neuromuscular Diseases
Motor Neuron Disease
Poliomyelitis
Virus Diseases
Central Nervous System Viral Diseases
Myelitis
Poliomyelitis
Poliomyelitis
RNA Virus Infections
Picornaviridae Infections
Enterovirus Infections
Poliomyelitis
Health Care
Health Care Facilities, Manpower, and Services
Health Personnel
Laboratory Personnel
Health Services Administration
Organization and Administration
Management Information Systems
Clinical Laboratory Information Systems
Information Science
Information Science
Medical Informatics
Medical Informatics Applications
Information Systems
Clinical Laboratory Information Systems
Management Information Systems
Clinical Laboratory Information Systems
Organisms
Viruses
RNA Viruses
Picornaviridae
Enterovirus
Polioviruses
Poliovirus
Vertebrate Viruses
RNA Viruses
Picornaviridae
Enterovirus
Polioviruses
Poliovirus
Persons
Persons
Occupational Groups
Health Personnel
Laboratory Personnel
Copyright Restrictions: None - This image is in the public domain and thus free of any copyright restrictions.