ID#: 10784
Caption:
This 1972 image depicts a Mueller-Hinton agar culture plate that had been used in an antibiotic susceptibility test (AST). Known as the Kirby-Bauer method, each of the small, labeled discs, or wafers, contained an antimicrobial agent. The light halos surrounding each disc, also known as reaction zones, represented regions in which the bacteria on the agar’s surface did not thrive, due to their sensitivity to the antibiotic that had been soaked into these respective discs. See PHIL 10787 and 10789 for magnified views of this photograph. These tests, which measure the presence or absence of a reaction zone, also take into account the pH of the medium, the concentration of the inoculum, and a number of other factors. Using this method, the sensitivity a bacterial strain has to a given antimicrobial, or combination of antimicrobials, may be determined.
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Content Provider(s): CDC/ Gilda L. Jones
Creation Date: 1972
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Copyright Restrictions: None - This image is in the public domain and thus free of any copyright restrictions.